The scripts
The following scripts are available:
organoid_tracker.py
- starts a graphical program, which is used to visualize all data.organoid_tracker_compare_positions.py
- compare two sets of position detection dataorganoid_tracker_compare_links.py
- compare two sets of linking data.organoid_tracker_create_links.py
- uses a cell positions file to link cells from different time points together.organoid_tracker_detect_gaussian_shapes.py
- uses the raw images and provided cell positions toorganoid_tracker_extract_validate_errors.py
- after you have corrected all the warnings, counts in how many cases a change was made to the tracking data.
The command-line scripts have documentation included inside themselves. The visual scripts are all essentially the same, and are documented in [VISUALIZER].
Running the scripts
You can always run a script using the Python command from an Anaconda terminal:
python <path/to/script_name>.py
Or, if you are fine with using your default Python 3 installation of your system, you can run the script as follows:
<path/to/script_name>.py
On Windows, replace “.py” by “.bat” or simply leave out the extension altogether: (“.bat” is one of the default file extensions). If you don’t want to always type out the complete path to the script, then you need to add the OrganoidTracker folder to your system’s PATH.